Reloj citoplasmatico y desarrollo embrionario temprano: un modelo con futuras aplicaciones medicas y comerciales.

Reloj citoplasmatico y desarrollo embrionario temprano: un modelo con futuras aplicaciones medicas y comerciales.

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Omar Camargo


El ciclo meiotico en los mamiferos comienza en el ovario fetal ,prosigue hasta el estado de diplonete de la primera profase, y se detiene , poco antes del nacimiento en una fase similar a G2.La reiniciacion  de la meiosis, la cual ocurre inmediatamente antes de la ovulacion, representa la transicion entre las fases G2 y M del ciclo celular.  

Una ves ovulado y fertilizado el oocito es activado, fenomeno que le capacita para iniciar el clivage el cual consiste en rapidas alteraciones de los estados de interfase y de mitosis.Se ha sugerido que un "reloj ordenador" controla el clivage post-cigotico en virtud a que estas divisiones son relativamente rapidas , sincronicas y no dependen del crecimiento celular.Aunque varios componentes de este reloj se han descubierto , los elementos que determinan su ciclidad no han sido identificados .El reloj ,se caracteriza por la actividad del factor promotor de la fase M(FPM), una cinasa dependiente de ciclinas ,que cuando se activa induce la celula a la mitosis .De otro lado se ha hallado que aumentos periodicos de la concentracion de calcio libre citosolico([Ca++]i)contribuye a la regulacion de  los ciclos celulares . Aunque el grado de regulacion ejercido por dichas oscilaciones de [Ca++]i no se conoce con certeza , se ha sugerido que el mecanismo que las controla es un componente fundamental del gran reloj ordenador.                                                                                                                                                                                          

Aqui exploramos las posibles interacciones que pueden existir entre Ca++ y el FPM,como estas regulan los ciclos celulares  durante el desarrollo embrionario temprano (post-cigotico)] y que aplicaciones medicas  y comerciales se han derivado de su estudio .

Palabras Clave: ciclo celular, ciclinas, cinasas -fosfatasas, activación, desarrollo temprano.


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Referencias (VER)

CAMBELL K.,LOI P., OTAEGUI P .and WILMUT I.Cell cycle cocoordination in embryo cloning by nuclear transfer .Rev .Reprod. 1996: I:40-46.

CARROLLO J.,JONES K And WHITTINGHAM D. Ca2 release and the development of Ca2+ release mechanisms during oocyte maturation: a prelude to fertilization .Rev.reprod. 1996;I:137-143.

CISEK L-and CORDEN J.Phosphorylation of RNA polymerase by the murine homologue of the cell-cycle control protein Cdc2.Nature 1989;339:679-684.

COLLAS R. and ROBL J. Factors affecting efficiency of nuclear transplantation in the rabbit.Biol.Reprod. 1990;43:877-884.

DAMIANI P.,FISSORE R.,CIBELLI J., LONG C., BALISE J., ROBL M. and DUBY R.Evaluation of developmental competence, nuclear and ooplasmic maturation of calf oocytes.Molec. Reprod .Devel.1996;45:521-534.

DEKEL N.Protein phosphorylation/ dephosohorylation in the meiotic cell cycle of mammalian oocytes.Rev . Reprod.1996;1:82-88.

EVANS T.,ROSENTHAL E.,YOUNGBLOM J.,DISTELD D. and HUNT T.Cyclin :a protein specified by maternal mRNA insea urchin eggs that is destroyed at each cleavage division .Cell 1883. 33;389-396.

FINKBEINER S.and GREENBERG E.Spatial features of calcium- regulated gene expression.Bio Essays 1997;19:657-660.

FISSORE RA.GORDO AC.And WU H.Activation of development in mammals : is there a role for a sperm cytosolic factor? Theriogenology 1998;49:43-52.

FISSORE R., LI HE C.and VANDE WOUDE F.Potential role of mitogen-activated protein kinase during meiosis resumption in bovine oocytes. Biol.Reprodu.1996;55:1261-1270.

GERHART J.,WU M. and KIRSCHNER M.Cell dynamics of an Mphase- specific cytoplasmatic factor in xenopus laevis oocytes anf eggs.

GILBERT SF.Developmental Biology . 4ed . Sinauer Associates Inc.Sunderland, Massachusetts:1994.

GLOTZER.,MURRAY A. and KIRSCHNER M.Cyclin is degrade by the ubiquitin pathway. Nature 1991;349:132-138.

GURLEY L.,D´anna J.BARHAM S.DEAVEN L.and TOBEY R.Histone phosphorylation and chromatin structure during mitosis in Chinese hamstercells . Eur.J. Biochem. 1992;84:1-15.

JEFFREY P.,RUSSO A., POLYAK K., GIBBS E.,HURWINTZ J., MASSAGUE N. and PAVLETICH N.Mechanism of CDK activation revealed by structure of a cyclin A-CDK complex Nature 1995:376:313-320.

JONES K., CARROLL J., MERIMAN J., WHITTINGHAMD, and KONO T.Repetitive sperm- induced Ca2+ transients in mouse oocytes are cell-cycle dependent . Development 1995;121:3259-3266.

KAO J.,ALDERTON J., TSIEN R.and STEINHARDT R.Active involvement of Ca2+in mitosis progression of swiss 3T3 fibroblasts. J .Cell Biol. 1990;111: 183-196.

KLINE D . Activation of the mouse egg.Theriogenology 1996;45:81-90.

KONO T.,JONES K., BOS-MIKICH A. WHITTINGHAN D.and CARROLLO J.A cell cycle-associated change in Ca2+ releasing activity leads to the generation of Ca2+ transients in mouse embryos during the first mitotic division. J .Cell Biol .1996;915 -923.

LAI F.,ERICKSON H.,ROUSSEAU E., LUI Q.and MEISSNER G. Purificacion and reconstitution of the calcium release channel from skeletal muscle .Nature 1988;331:315-319.

LAWRENCE Y.,WHITAKER M.and SWANN K. Sperm egg fusion is the prelude to the initial Ca2+ increase at fertilization in the mouse .Development 1997;124:233-241.

LEE M.and NURSE P.Complementation used to clone a human homologue of the fission yeast cell cycle control gene.Nature 1987;237:31-35.

LORCA T.,CRUZALEGUI F.FESQUET D., CADA VORE J. MERY J.,MEANS A. And DOREE M. Calmodulin –dependent protein kinase II mediates inactivation of MPF and CSF upon fertilization of Xenopus eggs.Nature. 1993;366:270-273.

MACHATY Z.,FUNAHASHI H.,MAYES M.,DAY B. and PRATHER R.Effects of injectin calcium chloride into in vitro-maturated porcine oocytes.Biol .Reprod.1996;54:316-322.

MASUI Y.and CLARKE R.Oocyte maturation.Inter,Rev.Cytol 1979;57:185-282.

MASUI Y.and MARKET C.Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.J .Exp. Zool.1971;177:129-146.

MINSHULL J.BLOW JJ. And HUNT T. Translation of cyclin mRNA is necessary for extracts of activated Xenopus eggs to enter mitosis.Cell 1992;56:947-956.

MIYAZAKI S.,YAZUKI M.,NAKADA K.,SHIRAKAWA H., NAKANISHI S., NAKADE S.and MIKOSHIBA K.Block of Ca2+ wave and Ca2+ oscillations by antibody to the inositol 1,4, 5, triphosphate receptor in fertilized hamster eggs.Science 1992;257:251-255.

MURRAY A., and HUNT T.The cell cycle an introduction ,1 st ed. 251 pp.W.H. Freeman &Co. New York; 1993.

MURRAY A.and KIRSCHNER M.Cyclin synthesis drives the early embryonic cell cycle.Nature 1989;339:275-280.

MYAKE-LYE R. and KIRSCHNER M.Induction of early mitotic events in a cell- free system. Cell 1985;41:165-175.

NEWOPORT JW.and KIRSCHNER MW.Regulation of the cell cycle during early Xenopus development.Cell 1984:37:731-742.

NURSE P.Universal control mechanism regulating onser of M-phase.Nature 1990;344:503-508.

OZIL J.The parthenogenetic development of rabbit oocytes after repetitive pulsatile electrical stimulation.Developmen 1990:109:117-127.

PALERMO G.,COLOMBERO L.And ROSENWAKS Z.,The human sperm centrosomeis responsible for normal syngamy and early embryonic development.Rev. Reprod. 1996;2 :19-27.

PARRINGTON J.,SWANN K., SHEVSHENKO V.,SESAY A. and LAI F.Calcium oscillations in mammalian eggs triggered by a soluble sperm protein.Nature 1996:379:364-368.

PINES J.and HUNTER T.Human cyclins A and B1 are differentially-located in the cell and undergo cell cycle –dependent nuclear transport.J .Cell Biol . 1991:115:1-17.

SATTERWHITEL L.,LOHKA M.,WILSON K., SCHERSON T.,CISEK L.and POLLARD T.phosphorylation of myosin-II light chain by cyclin-p34cdc2:A mechanism for the timing of cytokinesis. J.Cell Biol.1992;118:595-605.

SCHMIADI H. and KENTENICH H.Premature chromosome condensation after in vitro fertilization.Human.Reprod.1989;4:689-695.

SHIBUYA E.and MASUI Y.Molecular characteristics of cytostanic factors in amphibian egg extracs.Development 1989;106:799-808.

SIMERL Y C.,WU G-J.,ZORAN S.,et al. The paternal inheritance of the centrosome , the cell´s microtubule-organizing centre, in humans , and the implications for infertility.Nature Medicine 1995;47-52.

SWANSOS C.,ARKIN A.and ROSS J.An endongenous calcium oscillator may control early embryonic division.Proc.Natl.Acad.Sci.USA 1997;94:1194-1199.

TWING J.,PATEL R.and WHITAKER M.Translation control of InsP3-induced chromatin condensation during the early cell cycles of sea urchin embryos.Nature 1988;332:366-369.

VAN BLERKOM J,DAVIS P.and MERRIAM J. A retrospective analysis of unfertilized and presumed parthenogenetically activated human oocytes demonstrates a high frecuency of sperm penetration .Human Reprod.1994;9:2381-2388.

WHITAKER M. Control of meiotic arrest .Rev Reprod. 1996;1:127-135.

WHITAKER M.and PATEL R. Calcium and cell cycle control.Development 1990;108:525-542.

WHITE K.and YUE C.Intracellular receptors and agents that induce activation in bovine oocytes Theriogenology 1996;45:91-100.

WILMUT I.,SCHNEIKE AE.,McWHIR J.,KIND AJ., and CAMPBELL KHS .Viable offspring derived from fetal and adult mammalian cells. Nature 1997;285:810-813.

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