Evaluación de la motilidad espermática a través del sistema C.A.S.A de semen caprino criopreservado bajo diferentes medios diluyentes

  • Leonardo Hernández-Corredor Universidad Francisco de Paula Santander.
  • Alexander Nivia-Osuna UNAD
  • Daniel Hernández-Villamizar Universidad Francisco de Paula Santander, Ocaña.
  • Jorge Alexander Rubio-Parada Universidad Francisco de Paula Santander.
  • Armando Quintero-Moreno Universidad del Zulia, Maracaibo.

Resumen

 

El estudio evaluó la motilidad espermática y su efecto postdescongelación en semen caprino, en dos medios comerciales (Andromed® y TwoStep®) y diferentes protocolos de congelación (medio completo, con adicción del 10% de yema de huevo, semen centrifugado y sobrenadante seminal), se utilizaron machos de la raza alpina de la Universidad Francisco de Paula Santander Ocaña, el semen fue colectado con electroeyaculador, una vez los medios terminados y parte de los contenidos seminales enteros o centrifugados mezclados, se estabilizó por 2 horas, se envasó en pajillas de 0,5 cc y se congela en vapores de nitrógeno por 10 minutos, las pajillas se llevaron al laboratorio de Andrología de la Universidad del Zulia y por medio del sistema C.A.S.A.(Computer Assisted Sperm Análisis) se evaluaron los parámetros de motilidad como velocidad curvilínea (VCL), velocidad rectilínea (VSL), velocidad lineal (VAP), índice de linealidad (LIN), índice de rectitud (STR), índice de oscilación (ALH), Amplitud media del desplazamiento lateral de la cabeza del espermatozoide (BCF), los datos fueron analizados por medio del procedimiento GLM de SAS versión 9.0; los mejores índices de motilidad (VCL, ALH, BCF) fueron expresados enel tratamiento de contenido seminal centrifugado en medio Andromed®. (p≤0,001))La mejor progresividad espermática (VSL,LIN,STR)se presentó el tratamiento de Semen completo de caprino, criopreservado en medio comercial TwoStep®.

 

ABSTRACT

 

The study evaluated the effect sperm motility and sperm post-thawing in goats, two commercial means (Andromed ® and Two Step ®) and different freezing protocols (complete medium with 10% addition of the egg yolk, semen centrifuged supernatant and seminal ), we used males of the Alpine race of the University Francisco de Paula Santander Ocaña, semen was collected with electroejaculator once finished media and part of the whole and centrifuged seminal contents mixed, stabilized by two hours, packed in 0.5 cc straws and frozen in nitrogen vapor for 10 min, the straws were taken to the laboratory of Andrology at the University of Zulia and through CASA system (Computer Assisted Sperm Analysis) were evaluated motility parameters such as curvilinear velocity (VCL), straight line velocity (VSL), linear velocity (VAP), linearity index (LIN), straightness index (STR) Oscillation Index (ALH ) average amplitude of the lateral displacement of the sperm head (BCF), the data were analyzed by the GLM procedure of SAS version 9.0, the highest rates of motility (VCL, ALH, BCF) were expressed in the treatment of seminal content centrifugation Andromed ® medium. (p ≤ 0.001)) The best progressive sperm (VSL, LIN, STR) will present the full Semen treatment goats, cryopreserved at Two Step ® commercial medium.

Keywords: semen, buck, Andromed, Two step.

Biografía del autor/a

Leonardo Hernández-Corredor, Universidad Francisco de Paula Santander.
Magister en Ciencias Agrarias.
Alexander Nivia-Osuna, UNAD
Magister en Ciencias Veterinarias.
Daniel Hernández-Villamizar, Universidad Francisco de Paula Santander, Ocaña.
Magister en Producción Animal.
Jorge Alexander Rubio-Parada, Universidad Francisco de Paula Santander.
Magister en Ciencias Agrarias.
Armando Quintero-Moreno, Universidad del Zulia, Maracaibo.
Doctor en Ciencias Veterinarias.

Referencias bibliográficas

Amann, R. y Katz, D. 2004. Reflections on CASA after 25 years. J. Androl. 25:317–325.

Ávila-Portillo, L., Madero, J., López, C., León, M., Acosta, L.; Gómez, C.; Delgado, L.; Gómez, C., Lozano, J. y Reguero, M. 2006. Fundamentos de criopreservación. Revista Colombiana de Obstetricia y Ginecología Vol. 57 No. 4. (291-300).

Azerêdo, G., Esper, C. y Resende, K. 2001. Evaluation of plasma membrane integrity of frozen-thawed goat spermatozoa with or without seminal plasma. Small Rumin Res; 41:257– 63.

Bag, S.; Joshi, A.; Naqvi, S.; Rawat, P. y Mittal J. 2002. Effect of freezing temperature, at which straws were plunged into liquid nitrogen, on the post-thaw motility and acrosomal status of ram spermatozoa. Animal ReproductionScience 72: 175–183.

Bravo, J., Montanero, J., Calero, R. y Roy, T. 2011. Relación entre variables subjetivas e informatizadas del movimiento espermático del morueco. Arch. Zootec. 60 (232): 1087-1094.

Broekhuijse, M., Sostaric, E., Feitsma H. y Gadella M. 2012. Application of computerassisted semen analysis to explain variations in pig fertility. J ANIM SCI, 90:779-789.

Budworth, P. R., R. P. Amann, and P. L. Chapman. 1988. Relationships between computerized measurements of motion of frozen thawed bull spermatozoa and fertility. J. Androl. 9:41–54.

Cabrera, F., Gonzalez, F., Batista, M., Calero, P., Medrano, A. y Gracia A. 2005. The effect of removal of seminal plasma, egg yolk level and season on sperm freezability of canary buck (Caprahircus). Reprod.Domest. Anim;40:191–5.

Caiza de la Cueva FI; Rigau T.; Bonet S; MiroJ; Briz M.; Rodríguez J. 1997. Subjecting horse spermatozoa to hypoosmotic incubation: effects of ouabain. Theriogenology, 47: 765-784.

Camacho, O. 2011. Criocapacitación de espermatozoides caprinos, procesados con dos diluyentes. Tesis de grado. Universidad Veracruzana.54 p.

Castilho, E., Guimarães, J., Martins, L., Oliveira, R.; Facioni, S. y Borela C. 2009. Uso de própolis e ácido ascórbico na criopreservação do sêmen caprino. R. Bras. Zootec., v.38, n.12, p.2335-2345.

Cox, F.; Alfaro, V., Montenegro, V.; Rodriguez- Martinez, H. 2006. Computer-assisted analysis of sperm motion in goats and its relationship with sperm migration incervical mucus, Theriogenology, 66: 860–867.

Daskin, A., Kulaksiz, R., Akçay, E. y Erol, H. 2011. The Effect of Different Dilution Rates of Angora Buck Semen Frozen with Bioxcell Extender on the Post-thaw Quality. J Fac Vet Med UnivErciyes 8(1), 23-26.

Dott HM, Foster GC. The estimation of sperm motility in semen, on a membrane slide, bymeasuring the area change frequency with an image analyzing computer. J ReprodFertil 1979;55:161-166.

Dorado, J. 2003. Respuesta a la congelacióndescongelación del esperma de macho cabrío. Tesis doctoral. Universidad de Córdoba. Facultad de Veterinaria.

Dorado, J., Hidalgo, M., Muñoz, A. y Rodríguez, I. 2009. Assessment of goat semen freezability according to the spermatozoa characteristics from fresh and frozen samples. AnimalReproductionScience112 :150–157.

Dubeibe, D., Pinzón, B.; Salazar, P. y Serrano, C. 2007. Comparación de dos diluyentes para el congelamiento de semen caprino de la raza santandereana. Rev Col CiencPec; 20:4,535.

Flesh, F. y Gadella, B. 2000 Dynamics of the mammalian sperm membrane in the process of fertilization. Biochim.Biophys.Acta:197–235.

Flowers W.L. 1997. Management of boars for efficient semen production. J Reprod Fertil 52: 67-68.

Foxcroft, G., Dyck, M., Ruiz-Sanchez, A., Novak, S. y Dixon W. 2008. Identifying useable semen.Theriogenology, 70(8):1324-36.

Gacitua, H. y Arav, H.2005. Successful pregnancies with directional freezing of large volume buck semenTheriogenology, 63:931–938

Gravance CG, Davis RO. Automated Sperm Morphometry Analysis (ASMA) in the Rabbit. J. Androl. 1995, 16:88-93

Hirano, Y., H. Shibahara, H. Obara, T. Suzuki, S. Takamizawa, C. Yamaguchi, H. Tsunoda, and I. Sato. 2001. Relationships between sperm motility characteristics assessed by the computeraided sperm analysis (CASA) and fertilization rates in vitro. J.Assist.Reprod. Genet.18:213–218.

Januskauskas A., Johannisson A., y Rodriguez- Martinez H. 2003. Subtle membrane changes in cryopreserved bull semen in relation withsperm viability, chromatin structure, and field fertility. Theriogenology, 60:743–58.

Kathiravan, P., Kalatharan, J., Karthikeya, G., Rengarajan, K. y Kadirvel, G. 2010. Objective sperm motion analysis to assess dairy bull Fertility using computer-aided system – A review. Reprod.Domest.Anim; doi: 10.1111/j.1439-0531.2010. 01603.x

Karagiannidis, A.; Varsakeli, S. y Karatzas, G. 2000. Characteristics and seasonal variations in the semen of Alpine, Saanen and Damascus goat bucks born and raised in greeceTheriogenology, 53: 1285-1293.

Krishnakumara, S., Whitesideb, D., Elkinb, B. y Thundathila, J. 2011. Evaluation of ananimal protein-free semen extender for cryopreservation of epididymal sperm from NorthAmerican bison (Bison bison) Theriogenology, 76:252–260.

Kraemer, M., Fillion, C., Martin-Pont, B., Auger, J. 1998.Factors influencing human sperm kinematic measurements by the Celltrak computer-assisted sperm analysis. Human Reprod. 13: 611-9.

Lavara, R., E. Moce, F. Lavara, M. Pilar Viudes de Castro, and J. Salvador Vicente. 2005. Do parameters of seminal qualitycorrelate with the results of on-farm inseminations in rabbits? Theriogenology, 64:1130–1141.

Lavara, R. 2009. Estimación de los parámetros genéticos y calidad seminal de los parámetros genéticos de producción y calidad seminal en una línea paternal de conejos. Universidad Politecnica de Valencia, 67 p.

Leboeuf, B., Restall, B. y Salamon, S. 2000. Production and storage of goat semen for artificial insemination.Anim.Reprod.Sci; 62:113– 41.

Leite, T., Do Vale Filho V., De Arruda, R., De Andrade, A., Emerick, L., Zaffalon, F., Martins, J. y De Andrade, V. 2010. Effectsof extender and equilibration time on post-thaw motility and membrane integrity of cryopreserved Gyr bull semen evaluated by CASA and flow cytometry. Anim.Reprod.Sci;120: 31–8.

Le Grandois, J., Marchioni, E., Zhao, M., Giuffrida, F., Ennahar,S., y Bindler F.2009. Investigation of natural phosphatidylcholine sources: separation and identification by liquid chromatography-electrospray ionizationtandem mass spectrometry (LC-ESI-MS2). ofmolecular species. J Agric Food Chem 2009;57:6014 –20.

Marín-Briggiler, I., Tezón, J., Miranda, P., Vazquez-Levin, M. 2002. Effect ofincubating human sperm at room temperature on capacitation- related events. FertilSteril. 77: 252-59.

Mortimer, S. y Mortimer, D. 1990. Kinematics of human spermatozoa incubated under capacitating conditions. J. Androl. 11, 195.

Mortimer, D. 1994. Practical Laboratory Andrology. Oxford University press.Sydney IVF. Australia

Miro, J., Taberner. E., Rivera, M., Peña, A., Medrano, A., Rigau, T. y Peñalba, A.2009. Effects of dilution and centrifugation on the survival of spermatozoa and the structure of motile sperm cell subpopulations in refrigerated Catalonian donkey semen Theriogenology, 72:1017–22.

Nava, A. y Coronado, L. 2010. Comparación de dos Diluyentes de Base Tris (Hidroximetilaminometano) Sobre la Motilidad del Semen Caprino Congelado-Descongelado. Tesis UDO. Monagas. 49 P

Nordstoga, A., Söderquist, L., Ådnøy, T. y Paulenz, H. 2011. Fertility results after vaginal deposition of frozen-thawed buck semen diluted with two different extenders using one- or two-step procedures. Reprod.Domest. Anim;46:82– 6.

Palacios, L. y Wang, T. 2005. Egg-yolk lipid fractionation and lecithin characterization. J Am Oil Chem Soc.;82:571– 8.

Pellicer-Rubio, M., Magallon, T. y Combarnous Y. 1997. Deterioration of goat sperm viability in milk extenders is due to a bulbourethral-kilodalton glycoprotein with triglyceride lipase activity.Biol.Reprod;57:1023–31.

Pellicer-Rubio M. y Combarnous Y. 1998. Deterioration of goat spermatozoa in skimmed milk-based extenders as a result of oleicacid released by the bulbourethral lipase BUSgp60. J Reprod Fertil;112:95–105.

Peña, A. y Linde-Forsberg, C. 2000. Effects of Equex, one or two step dilution and twofreezing thawing rates on post-thaw survival of dog spermatozoa, Theriogenology, 5: 859–875.

Peterson, K., Kappen, M., Ursem, P., Nothling, J., Colenbrander, B. y Gadella, M. 2007. Microscopic and flow cytometric semen assessment of Dutch AI-bucks: effect of semen processing procedures and their correlation to fertility. Theriogenology, 67: 863–71.

Purdy, P. 2006. A review on goat sperm cryopreservation. Small Rumin Res;63:215–25. Quintero-Moreno, A., Miró, J., Rigau, T., Rodríguez-Gil, J.E.2003 Identification of sperm subpopulations with specific motility characteristics in stallion ejaculates. Theriogenology, :1973-1990.

Quintero-Moreno A., Rubio-Guillen J. 2008. Evaluación de la calidad espermática en toro mediante tecnología informática. Desarrollo Sostenible de la Ganadería de Doble Propósito. Capítulo LVIII, 707 714.

Quintero-Moreno A., González D., Garde J.J., Esteso M., Fernández-Santos MR., Carvalho JL., Mejía W., León G. 2009. Valoración Morfométrica de la cabeza del espermatozoide del cerdo doméstico según su edad. Revista Científica, FCV-LUZ, XIX: 153-158.

Quintero-Moreno, A., Rubio-Guillén J., González- Villalobos D., Gutiérrez JC., Madrid- Bury N.y López-Brea JJ. 2011. Identification of cryodamage on plasma membrane Integrity in bull spermatozoa and its relationship With field fertility. Revista Científica, FCV-LUZ, Vol. XXI, Nº 5, 403 – 407.

Roof, D., Bowley, S., Price, L. y Matsas, D. 2012.Comparison of two commercial extenders for cryopreservation ofgoat semen without sperm washing. Theriogenology, 77:412–420.

Samper, J., Hellander, J. yCrabo.B. 1991. Relationshipbetween the fertility of fresh and frozen stallion semen and semenquality. J. Reprod. Fertil. Suppl. 44:107–114.

Sariozkan, S., Bucak, M., Tuncer, P., Tasdemir, U., Kinet, H. y Ulutas, A. 2010. Effects of different extenders and centrifugation/washing on postthaw microscopic-oxidative stress parameters and fertilizing ability of Angora buck sperm. Theriogenology, 73:316 –23.

Sias, B., Ferrato, F., Pellicer-Rubio, M,,Forgerit, Y., Guillouet, P., Leboeuf, B. y Carriere, F. 2005. Cloning and seasonal secretion of the pancreatic lipase-related protein 2 present in goat seminal plasma.BiochimBiophys Acta; 1686:169–80.

Silvestre, F., Bezerra, T., Castelo, A., Oliveira, R., Lima, G., Peixoto, G., Bezerra, A., Silva.A. 2011. Objective assessment of the cryoprotective effects of dimethylformamide for freezing goat semen. Cryobiology 63: 263–266.

Tardif, S., Laforest J.P., Cormier N., Bailey J.L. 1999.The importance of porcine sperm parameters on fertility in vivo. Therigenology, 52:-459.

Tomas, C. 2007. Nuevos protocolos para la crioconservación de espermatozoides de macho cabrío. Universidad Politécnica de Valencia, España, 46 p.

Valencia, J.; González, G., González, M. y Trejo, A. 1994. Motilidad y daño acrosomal del semen caprino congelado en pajillas de 0,25 ml y 0,5 ml y descongelado a dos diferentes ritmos de temperatura. VetMéx., 25 (2) 127-131.

Vyt, P., D. Maes, C. Quinten, T. Rijsselaere, W. Deley, M. Aerts, A.de Kruif, and A. van Soom. 2008. Detailed motility examination of porcine semen and its predictive value towards reproductive performance in sows.

VlaamsDiergeneeskd. Tijdschr. 77:291–298. Vallecillo, A.; Trigo, P.; Delgado, J.; Cabello, A.; Santos, E. y Tenorio, T. 2004. effects of cryopreservation on sperm motility in blancaserranaandaluza goat. South African Journal of Animal Science, 34: 116-118.

White, D., Phillips, M., Bedford, M. 1990. Factorsaffecting the acrosome reaction in hu-man spermatozoa. J ReprodFertil. 90: 71-80.

Zarazaga, L.; Guzman, J.; Dominguez, C.; Perez, M. y Prieto R. 2009. Effects of season and feeding level on reproductive activity and semen quality in Payoya buck goats. Theriogenology, 71:1316–1325.

Cómo citar
Hernández-Corredor, L., Nivia-Osuna, A., Hernández-Villamizar, D., Rubio-Parada, J. A., & Quintero-Moreno, A. (2013). Evaluación de la motilidad espermática a través del sistema C.A.S.A de semen caprino criopreservado bajo diferentes medios diluyentes. Respuestas, 18(2), 16-27. https://doi.org/10.22463/0122820X.443

Descargas

La descarga de datos todavía no está disponible.
Publicado
2013-07-01
Sección
Artículos