Motility sperm evaluation system through c.a.s.a goat semen under different media cryopreserved diluents

Evaluación de la motilidad espermática a través del sistema C.A.S.A de semen caprino criopreservado bajo diferentes medios diluyentes

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Leonardo Hernández-Corredor
Alexander Nivia-Osuna
Daniel Hernández-Villamizar
Jorge Alexander Rubio-Parada
Armando Quintero-Moreno
Abstract

The study evaluated the effect sperm motility and sperm post-thawing in goats, two commercial means (Andromed ® and Two Step ®) and different freezing protocols (complete medium with 10% addition of the egg yolk, semen centrifuged supernatant and seminal ), we used males of the Alpine race of the University Francisco de Paula Santander Ocaña, semen was collected with electroejaculator once finished media and part of the whole and centrifuged seminal contents mixed, stabilized by two hours, packed in 0.5 cc straws and frozen in nitrogen vapor for 10 min, the straws were taken to the laboratory of Andrology at the University of Zulia and through CASA system (Computer Assisted Sperm Analysis) were evaluated motility parameters such as curvilinear velocity (VCL), straight line velocity (VSL), linear velocity (VAP), linearity index (LIN), straightness index (STR) Oscillation Index (ALH ) average amplitude of the lateral displacement of the sperm head (BCF), the data were analyzed by the GLM procedure of SAS version 9.0, the highest rates of motility (VCL, ALH, BCF) were expressed in the treatment of seminal content centrifugation Andromed ® medium. (p ≤ 0.001)) The best progressive sperm (VSL, LIN, STR) will present the full Semen treatment goats, cryopreserved at Two Step ® commercial medium.

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Author Biographies (SEE)

Leonardo Hernández-Corredor, Universidad Francisco de Paula Santander.

Magister en Ciencias Agrarias.

Alexander Nivia-Osuna, UNAD

Magister en Ciencias Veterinarias.

Daniel Hernández-Villamizar, Universidad Francisco de Paula Santander, Ocaña.

Magister en Producción Animal.

Jorge Alexander Rubio-Parada, Universidad Francisco de Paula Santander.

Magister en Ciencias Agrarias.

Armando Quintero-Moreno, Universidad del Zulia, Maracaibo.

Doctor en Ciencias Veterinarias.
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