Evaluación de la inducción de embriogénesis somática a partir de hojas cotiledonares de curuba de castilla (Passiflora mollissima) L.H Bailey
Evaluation of induction of somatic embryogenesis from cotyledonary leaves of Banana Passion fruit (Passiflora mollissima) L.H Bailey
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Parra Peñalosa, O. J. ., & Cancino Escalante, G. O. . (2019). Evaluación de la inducción de embriogénesis somática a partir de hojas cotiledonares de curuba de castilla (Passiflora mollissima) L.H Bailey. Respuestas, 24(3), 31–38. https://doi.org/10.22463/0122820X.1847
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Passiflora mollissima L.H Bailey es una especie endémica de los Andes de Colombia sus frutos son comestibles y presentan propiedades medicinales. El desarrollo de técnicas de micropropagación permiten proporcionar una propagación clonal rápida y eficiente de genotipos de élite con alta resistencia y producción uniforme, así como un sistema que pueda usarse para la transformación genética. Por esta razón, la investigación se centró en la evaluación de la inducción de embriogénesis somática en P. mollissima a partir de hojas cotiledonarias, el efecto de las concentraciones del regulador de crecimiento y la orientación de los explantes en la producción de embriones. Los análisis histológicos de la embriogénesis somática se realizaron cada 10 días después de la inducción durante más de 38 días de exposición al medio. Los resultados mostraron la formación de embriones somáticos en el medio de cultivo Murashige y Skoog, (1962) suplementado con ácido 2,4-diclorofenoxiacético 4,5 μM más 6-benciladenina 4,5 μM. Los alcances de esta investigación son aplicables al conocimiento de la embriogénesis no cigótica en las pasifloras de la región andina, con fines de mejora y uso comercial. Es de destacar que este es el primer estudio en la inducción y obtención de embriones en P. mollissima.
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R.C. Correa, R.M. Peralta, C.W. Haminiuk, G.M. Maciel, A. Bracht and I.C. Ferreira, “The past decade findings related with nutritional composition, bioactive molecules and biotechnological applications of Passiflora spp. (passion fruit)”, Trends in Food Science and Technology, Vol. 58, pp. 79–95, dic, 2016.
M. Lobo and C.I. Medina, “Cultivo, Poscosecha y Comercialización de las Pasifloráceas en Colombia: Maracuyá, Granadilla, Gulupa y Curuba”, Sociedad Colombiana de Ciencias Hortícolas, Bogotá, Colombia, 2009.
A.C. Salazar, C. Ramírez, “Fruit maturity stage and provenance affect seed germination of Passiflora mollissima (banana passion fruit) and P. ligularis (sweet granadilla), two commercially valuable tropical fruit species”, Seed Science and Technology, Vol. 45, No. 2, pp. 383–397, jul 2017.
M.V. Pires, A.A. de Almeida, A.L. de Figueiredo, F.P. Gomes and M.M. Souza Germination and seedling growth of ornamental species of Passiflora under artificial shade, Acta Scientiarum Agronomy, Vol. 34 No. 1, pp. 67-75, feb, 2012.
M. Delanoy, P. Van Damme, X. Scheldeman and J. Beltran, “Germination of Passiflora mollissima (Kunth) L.H.Bailey, Passiflora tricuspis Mast. and Passiflora nov sp. seeds”, Scientia Horticulturae, Vol. 110, No. 2, pp. 198–203, oct 2006.
M. Ozarowski and B. Thiem, “Progress in micropropagation of Passiflora spp. to produce medicinal plants: A mini-review”, Brazilian Journal of Pharmacognosy, Vol. 23, No. 6, pp. 937-947, nov 2013.
F. Nakayama, “Cultivo in vitro de tejidos de Passiflora caerulea”, Revista de la Facultad de Agronomía de la Universidad Nacional de La Plata, Vol. 42, No. 1, pp. 63–74, 1966.
M.J. Moran-Robles, “In vitro vegetative multiplication of axillary buds of P.edulis var. flavicarpa Degener and P. mollissima Bairley, Fruits, Vol. 33, pp. 701–715, 1978.
G. Pacheco, M. Simão and M.G. Vianna, “In vitro conservation of Passiflora A review”, Scientia Horticulturae, Vol. 211, pp. 305-311, nov 2016.
M. L. da Silva, D. L.P Pinto, M. P. Guerra, E.I.S. Floh, C. H. Bruckner and W.C. Otoni,”A novel regeneration system for a wild passion fruit species (Passiflora cincinnata Mast.) based on somatic embryogenesis from mature zygotic embryos”, Plant Cell, Tissue and Organ Culture, Vol. 99, No. 1, pp. 47–54, oct 2009.
D.L.P. Pinto, A.M.R. de Almeida, M.M. Rêgo, M.L. da Silva, E.J. de Oliveira and W.C. Otoni, “Somatic embryogenesis from mature zygotic embryos of commercial passionfruit (Passiflora edulis Sims) genotypes,” Plant Cell, Tissue and Organ Culture, Vol. 107, No. 3, pp. 521–530, jul 2011.
D.I. Rocha, L.M. Vieira, F.A.O. Tanaka, L.C. da Silva and W.C. Otoni, “Somatic embryogenesis of a wild passion fruit species Passiflora cincinnata Masters: histocytological and histochemical evidences”, Protoplasma, Vol. 249, No. 3, pp. 747–758, 2012.
Y.B. Rosa, M.C. Dornelas, “In vitro plant regeneration and de novo differentiation of secretory trichomes in Passiflora foetida L. (Passifloraceae)”, Plant Cell, Tissue and Organ Culture, Vol. 108 No. 1, pp. 91-99, jan 2012.
D. de Oliveira Prudente, R. Paiva, S. Carpentier, R. Swennen, F. Nery, S. L. Silva and B. Panis, “Characterization of the formation of somatic embryos from mature zygotic embryos of Passiflora ligularis Juss” Plant Cell, Tissue and Organ Culture, Vol. 131, No. 1, pp.: 97–105, jul 2017.
S. Khadke, A. Kuvalekar, “Direct somatic embryogenesis and plant regeneration from leaf and stem explants of Nothapodytes foetida: a critically endangered plant species”, International Journal of Plant, Animal and Environmental Sciences, Vol. 3, No. 1, pp. 257–264, jan 2013.
T. Murashige, F. Skoog, “A revised medium for rapid growth and bioassays with tobacco tissue culture”, Physiologia Plantarum, Vol. 15, pp. 473-479, 1962.
C.A. Schneider, W.S. Rasband and K.W. Eliceiri,” NIH Image to ImageJ: 25 years of image analysis”, Nature Methods, Vol 9, No. 7, pp. 671, jul 2012.
T.P. O’Brien, N. Feder and M.E. McCully, “Polychromatic staining of plant cell walls by toluidine blue O.”, Protoplasma, Vol. 59, No. 2, pp. 368–373, jul 1964.
T. O’Brien and M. McCully, The study of plant structure principles and selected methods, Melbourne: Termarcarphi Pty Ltd, 1981.
P. Deo, A. Tyagi, M. Taylor, R. Harding and J. Becke, “Factors affecting somatic embryogenesis and transformation in modern plant breeding”, The South Pacific Journal of Natural and Applied Sciences, Vol. 28, No. 1, pp. 27-40, 2010.
I. Bermúdez, T.D.S. Blanco, J. Pérez, L. García, N. Veitía and R. Collado, “Efecto de la orientación y la longitud del cotiledón inmaduro sobre la formación de embriones somáticos en dos genotipos cubanos de soya, Biotecnología Vegetal, Vol. 10, No. 2, pp. 121 - 128, apr 2010.
M. Griga, “Some factors affecting somatic embryogenesis efficiency in soybean (Glycine max [L.] Merrill)”, Biologia Plantarum, Vol. 35, No. 2, pp. 101-106, 1993.
J. Aparecida, M.L. Carneiro, I. Olívio and B. Appezzato “Anatomical study of somatic embryogenesis in Glycine max (L.) Merrill”, Brazilian Archives of Biology and Technology, Vol. 45, No. 3, pp. 277-286, sept 2002.
M. Ziv and J. Chen, The anatomy and Morphology of Tissue Cultured Plants. In: George EF, Hall MA, Jan De Klerk, G (Eds) Plant Propagation by Tissue Culture, Springer. Dordrecht, 2008.
G. Ranjan “In vitro somatic embryogenesis in callus cultures of Azadirachta indica A. Juss. A multipurpose tree”, Journal Forest Research, Vol. 10, No. 1, pp. 263-267 2005.
S. Vila, H. Rey, A. Gonzalez and L. Mroginski “Somatic embryogenesis and plant regeneration in Cedrela fissilis”, Biologia Plantarum, Vol. 53, No. 2, pp. 382-386, jun 2009
P. Das, “In Vitro somatic embryogenesis in some oil yielding tropical tree species”, American Journal of Plant Science, Vol. 2, No. 1, pp. 217-222, jun 2011.
D.K. Das and A. Rahman, “Induction of somatic embryogenesis and long-term maintenance of embryogenic lines of litchi”, Current Trends in Biotechnology and Pharmacy, Vol. 7, No. 2, pp. 625-634, apr 2013
G.M. da Silva, A. Cruz, W. Otoni, T. Pereira, D. Rocha and M. da Silva, “Histochemical evaluation of induction of somatic embryogenesis in Passiflora edulis Sims (Passifloraceae)”, In vitro Cellular & Developmental Biology - Plant, Vol. 51, No. 5, pp. 539-545, oct 2015.
S. Bernal, L. Duarte, D. Rodríguez, D.L. Bohórquez, E. Araque and J. Pacheco, “Embriogénesis no zigótica en Passiflora maliformis Nonzygotic Embryogenesis in Passiflora maliformis”, Revista Peruana de Biología, Vol. 25, No. 3, pp. 281- 290, jul 2018.
P. Sharma and M.V. Rajam, “Genotype, explant and position effects on organogenesis and somatic embryogenesis in eggplant (Solanum melongena L.)”, Journal of Experimental Botany, Vol. 46, No. 28, pp. 153-141, jan 1995.
T. Radhakrishnan, T.G.K. Murthy, K. Chandran and A. Bandyopadhyay, “Somatic embryogenesis in Arachishypogea: revisited” Australian Journal of Botany, Vol. 49, No. 6, pp. 753-759, 2001.
C.K. Kim, J.D. Chung, S.O. Jee and J.Y. Oh, “Somatic embryogenesis from in vitro grown leaf explants of Rosa hybrida L.”, Journal of Plant Biotechnology, Vol. 5, No. 3 169-172, mar 2003R.
Zegzouti, M.F. Arnold and J.M. Favre. “Histological investigation of the multiplication steps in secondary somatic embryogenesis of Quercus robur L”, Annals of Forest Science, Vol. 58, No. 6, pp. 681-690, jan 2001.
D.L. Paim Pinto, A.M. Almeida, M.M. Rêgo, M.L. Silva, E.J. Oliveira, W.C. Otoni. “Somatic embryogenesis from mature zygotic embryos of commercial passionfruit (Passiflora edulis Sims) genotypes”, Plant Cell Tissue and Organ Culture, Vol. 107, No. 3, pp. 521–530, jul 2011.
R.C. Correa, R.M. Peralta, C.W. Haminiuk, G.M. Maciel, A. Bracht and I.C. Ferreira, “The past decade findings related with nutritional composition, bioactive molecules and biotechnological applications of Passiflora spp. (passion fruit)”, Trends in Food Science and Technology, Vol. 58, pp. 79–95, dic, 2016.
M. Lobo and C.I. Medina, “Cultivo, Poscosecha y Comercialización de las Pasifloráceas en Colombia: Maracuyá, Granadilla, Gulupa y Curuba”, Sociedad Colombiana de Ciencias Hortícolas, Bogotá, Colombia, 2009.
A.C. Salazar, C. Ramírez, “Fruit maturity stage and provenance affect seed germination of Passiflora mollissima (banana passion fruit) and P. ligularis (sweet granadilla), two commercially valuable tropical fruit species”, Seed Science and Technology, Vol. 45, No. 2, pp. 383–397, jul 2017.
M.V. Pires, A.A. de Almeida, A.L. de Figueiredo, F.P. Gomes and M.M. Souza Germination and seedling growth of ornamental species of Passiflora under artificial shade, Acta Scientiarum Agronomy, Vol. 34 No. 1, pp. 67-75, feb, 2012.
M. Delanoy, P. Van Damme, X. Scheldeman and J. Beltran, “Germination of Passiflora mollissima (Kunth) L.H.Bailey, Passiflora tricuspis Mast. and Passiflora nov sp. seeds”, Scientia Horticulturae, Vol. 110, No. 2, pp. 198–203, oct 2006.
M. Ozarowski and B. Thiem, “Progress in micropropagation of Passiflora spp. to produce medicinal plants: A mini-review”, Brazilian Journal of Pharmacognosy, Vol. 23, No. 6, pp. 937-947, nov 2013.
F. Nakayama, “Cultivo in vitro de tejidos de Passiflora caerulea”, Revista de la Facultad de Agronomía de la Universidad Nacional de La Plata, Vol. 42, No. 1, pp. 63–74, 1966.
M.J. Moran-Robles, “In vitro vegetative multiplication of axillary buds of P.edulis var. flavicarpa Degener and P. mollissima Bairley, Fruits, Vol. 33, pp. 701–715, 1978.
G. Pacheco, M. Simão and M.G. Vianna, “In vitro conservation of Passiflora A review”, Scientia Horticulturae, Vol. 211, pp. 305-311, nov 2016.
M. L. da Silva, D. L.P Pinto, M. P. Guerra, E.I.S. Floh, C. H. Bruckner and W.C. Otoni,”A novel regeneration system for a wild passion fruit species (Passiflora cincinnata Mast.) based on somatic embryogenesis from mature zygotic embryos”, Plant Cell, Tissue and Organ Culture, Vol. 99, No. 1, pp. 47–54, oct 2009.
D.L.P. Pinto, A.M.R. de Almeida, M.M. Rêgo, M.L. da Silva, E.J. de Oliveira and W.C. Otoni, “Somatic embryogenesis from mature zygotic embryos of commercial passionfruit (Passiflora edulis Sims) genotypes,” Plant Cell, Tissue and Organ Culture, Vol. 107, No. 3, pp. 521–530, jul 2011.
D.I. Rocha, L.M. Vieira, F.A.O. Tanaka, L.C. da Silva and W.C. Otoni, “Somatic embryogenesis of a wild passion fruit species Passiflora cincinnata Masters: histocytological and histochemical evidences”, Protoplasma, Vol. 249, No. 3, pp. 747–758, 2012.
Y.B. Rosa, M.C. Dornelas, “In vitro plant regeneration and de novo differentiation of secretory trichomes in Passiflora foetida L. (Passifloraceae)”, Plant Cell, Tissue and Organ Culture, Vol. 108 No. 1, pp. 91-99, jan 2012.
D. de Oliveira Prudente, R. Paiva, S. Carpentier, R. Swennen, F. Nery, S. L. Silva and B. Panis, “Characterization of the formation of somatic embryos from mature zygotic embryos of Passiflora ligularis Juss” Plant Cell, Tissue and Organ Culture, Vol. 131, No. 1, pp.: 97–105, jul 2017.
S. Khadke, A. Kuvalekar, “Direct somatic embryogenesis and plant regeneration from leaf and stem explants of Nothapodytes foetida: a critically endangered plant species”, International Journal of Plant, Animal and Environmental Sciences, Vol. 3, No. 1, pp. 257–264, jan 2013.
T. Murashige, F. Skoog, “A revised medium for rapid growth and bioassays with tobacco tissue culture”, Physiologia Plantarum, Vol. 15, pp. 473-479, 1962.
C.A. Schneider, W.S. Rasband and K.W. Eliceiri,” NIH Image to ImageJ: 25 years of image analysis”, Nature Methods, Vol 9, No. 7, pp. 671, jul 2012.
T.P. O’Brien, N. Feder and M.E. McCully, “Polychromatic staining of plant cell walls by toluidine blue O.”, Protoplasma, Vol. 59, No. 2, pp. 368–373, jul 1964.
T. O’Brien and M. McCully, The study of plant structure principles and selected methods, Melbourne: Termarcarphi Pty Ltd, 1981.
P. Deo, A. Tyagi, M. Taylor, R. Harding and J. Becke, “Factors affecting somatic embryogenesis and transformation in modern plant breeding”, The South Pacific Journal of Natural and Applied Sciences, Vol. 28, No. 1, pp. 27-40, 2010.
I. Bermúdez, T.D.S. Blanco, J. Pérez, L. García, N. Veitía and R. Collado, “Efecto de la orientación y la longitud del cotiledón inmaduro sobre la formación de embriones somáticos en dos genotipos cubanos de soya, Biotecnología Vegetal, Vol. 10, No. 2, pp. 121 - 128, apr 2010.
M. Griga, “Some factors affecting somatic embryogenesis efficiency in soybean (Glycine max [L.] Merrill)”, Biologia Plantarum, Vol. 35, No. 2, pp. 101-106, 1993.
J. Aparecida, M.L. Carneiro, I. Olívio and B. Appezzato “Anatomical study of somatic embryogenesis in Glycine max (L.) Merrill”, Brazilian Archives of Biology and Technology, Vol. 45, No. 3, pp. 277-286, sept 2002.
M. Ziv and J. Chen, The anatomy and Morphology of Tissue Cultured Plants. In: George EF, Hall MA, Jan De Klerk, G (Eds) Plant Propagation by Tissue Culture, Springer. Dordrecht, 2008.
G. Ranjan “In vitro somatic embryogenesis in callus cultures of Azadirachta indica A. Juss. A multipurpose tree”, Journal Forest Research, Vol. 10, No. 1, pp. 263-267 2005.
S. Vila, H. Rey, A. Gonzalez and L. Mroginski “Somatic embryogenesis and plant regeneration in Cedrela fissilis”, Biologia Plantarum, Vol. 53, No. 2, pp. 382-386, jun 2009
P. Das, “In Vitro somatic embryogenesis in some oil yielding tropical tree species”, American Journal of Plant Science, Vol. 2, No. 1, pp. 217-222, jun 2011.
D.K. Das and A. Rahman, “Induction of somatic embryogenesis and long-term maintenance of embryogenic lines of litchi”, Current Trends in Biotechnology and Pharmacy, Vol. 7, No. 2, pp. 625-634, apr 2013
G.M. da Silva, A. Cruz, W. Otoni, T. Pereira, D. Rocha and M. da Silva, “Histochemical evaluation of induction of somatic embryogenesis in Passiflora edulis Sims (Passifloraceae)”, In vitro Cellular & Developmental Biology - Plant, Vol. 51, No. 5, pp. 539-545, oct 2015.
S. Bernal, L. Duarte, D. Rodríguez, D.L. Bohórquez, E. Araque and J. Pacheco, “Embriogénesis no zigótica en Passiflora maliformis Nonzygotic Embryogenesis in Passiflora maliformis”, Revista Peruana de Biología, Vol. 25, No. 3, pp. 281- 290, jul 2018.
P. Sharma and M.V. Rajam, “Genotype, explant and position effects on organogenesis and somatic embryogenesis in eggplant (Solanum melongena L.)”, Journal of Experimental Botany, Vol. 46, No. 28, pp. 153-141, jan 1995.
T. Radhakrishnan, T.G.K. Murthy, K. Chandran and A. Bandyopadhyay, “Somatic embryogenesis in Arachishypogea: revisited” Australian Journal of Botany, Vol. 49, No. 6, pp. 753-759, 2001.
C.K. Kim, J.D. Chung, S.O. Jee and J.Y. Oh, “Somatic embryogenesis from in vitro grown leaf explants of Rosa hybrida L.”, Journal of Plant Biotechnology, Vol. 5, No. 3 169-172, mar 2003R.
Zegzouti, M.F. Arnold and J.M. Favre. “Histological investigation of the multiplication steps in secondary somatic embryogenesis of Quercus robur L”, Annals of Forest Science, Vol. 58, No. 6, pp. 681-690, jan 2001.
D.L. Paim Pinto, A.M. Almeida, M.M. Rêgo, M.L. Silva, E.J. Oliveira, W.C. Otoni. “Somatic embryogenesis from mature zygotic embryos of commercial passionfruit (Passiflora edulis Sims) genotypes”, Plant Cell Tissue and Organ Culture, Vol. 107, No. 3, pp. 521–530, jul 2011.
