Ensayos para la estandarizacion de una técnica de fenotipificacion de C4 del complemento serico.
Ensayos para la estandarizacion de una técnica de fenotipificacion de C4 del complemento serico.
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Como parte del proceso de caracterización genética de la población Colombiana, el Instituto de Genética Humana de la Universidad Javeriana, ha venido desarrollando investigaciones sobre diversos polimorfismos de proteínas séricas , tales como: Haptoglobinas, fosfoglucomutasa, componente del grupo específico , C3 (Sarmiento, P, 1993), Transferrina (Ortiz, R, 1993), enzimas critocitarias, proteínas de membrana y
marcadores moleculares (Bernal, J, 1989). Como parte de este proceso, en este estudio se estandarizó la técnica de Electroforesis de alto voltaje e lnmunolijación de C4 lo que permitirá incluir esta proteína en los estudios de población en Colombia.
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ALARCON V.GILBERTO .Estudio inmunologico de pacientes mesticos mexicanos con espondilocartropatias .Mex.1988,pp 118-46.
ALPER .C, and JOHNSON A. Immunofixation electrophoresis.A technique for the snady of protein polymorphism. Vox Sang 17.1969.pp 445-452.
ALVIN D.The efficiency of complement activation in MHC linked diseases Immunology Today .Vol 4,n9,1983.
AVERILI ,B. And BERNAL,J. Genetic and environmental influences on serum levels of human C4.Annals of Human Biology.Vol.11 n2,1984,149-156.
AVILA L. y ROMERO C.Manual Practico de Inmunologia clinica .I. M.Editores primera edicion Bogota ,1995.
AWDER, Z. And ALPER C.Genetic polymorphis ;of human complements C4 and detection of heterozygotes .Nature Vol 282,1979,pp 205-207.
AWDER, Z. And ALPER C.Inherited structural polymorphysm of the fourth component of human complement .Proc .Nad.Acad Sci USA Vo 77, n6,1980,pp 3576-3580.
BECKER,P.Genetica Humana ediciones Toray .Barcel.1980,pp 214-235.
BERG, S. Et al .Use of DNA amplification(PCR) and direct DNA sequencing in the characteriterizacion of C4 alleles .Ann Hum Genet 54,1990.pp 183-189.
BERNAND .H. Diagnostico y tratamiento clinico por los laboratorio.Octava edition ,edicion Salvat ,Barcelona ,1988.pp 1081-1096.
BERNAL,I, et al.Complement polymorphism in Colombia .Annals of Human Biology .vol 12,n3,1985.pp 261-265.
BERNAL,I,AVERILL ,B and SARMIENTO ,P. Genetic and environmental influences on serum levels of human complement components C4 and Bf in Colombian families .Annals Human Biology .1984.
BLAKE ,N. and RADFORD . P. Detection of chromosome 6 Non-HLA gene products .Human Disease .1984,pp 52-64.
BRAUN, L. es al.Null alleles of human complement C4. J. Exp. Med 171,1990,pp.129-140.
BRUUN P.,et al .Family studiens of complement C4 and HLA in man .Hum .Gent ,58,1981,pp.260-267. BRUUN P.,Et al. Genetics of complement C4.Two homoduplication haplotypes C4S and C4F in a family .Hum Genet .61,1982,pp.36-38.
BUETTNER J. Antropología Fisica Editorial Limusa ,Mexico 1980,pp 413-448.
CAMERON.P.,et al. Major histocompability complex genes influence the outcome of HIV infection ancestral haplotypes with C4 null alleles explain dverse HLA associations Human Immunology :29,1990.pp 282-295.
CARROL L ,M.Et al . A molecular map of the human mayor histocompatibility complex ciass III region linking complementogenes C4,C2 and factor B,Nature , vol 307,1984,pp 237-241.
CHAKRA VORTI. N, et al .The chemical structure of the C4d fragment of the human complement component C4. Molecular Immunology, vol 24,n 11,1987.pp 1187-1197.
CHAN, A . and ATKINSON , J. Oligosacharide structure of human C4.Journal of Immunology ,vol 134,n 3,1985,pp 1790-1798.
CHRISPEELS, J, et al. Sandwich enzymelinked immunosorbent assays for the quantification of the isotypes C4(C4 and C4B)in human plasma .Journal Immunological Methods, 125.1989,pp.5-12.
DODDS S.Et al.The purification and propertier of some less common allotypes of the fourth component of human complement. Immunogenetics 24,1986.pp 279 -285.
DOXIADIS,G.and GROSSE-WILDE H.C4 allotiping by prolonged gel electrophoresis an immunoblotting by prolonged gel electrophoresis and immunoblotting using monoclonal and polyclonal antibodies .Complement Inflamm 7,1990.pp 269-276.
FRIEDEMAN N. Y otros America Negra .Pontificia Universidad Javeriana, n4,1992. Bogota.
GILES, C.A new genetic variant for Chido. Vox Sang 1984-,pop 149-156.
GILES C. et al Antingenic determinants expressed by human allotypes; a, study of 325 families provides evidence for the structural antigenic model.Immunogenetic 27, 1988. pp 442—448.
GILES C. Et al C4 and HLA haplotypes associated with partial inhibition of anti — RG and anti - Ch journal of immunogenetics, II, 1984, 305—317.
GILES C. et al . The study of a French family with two duplicated C4A haplotypes Human Genetics, vol 77, 1987, pp. 359— 365.
GORGL Y. et al. A new duplication C4B*l.12 at the C4B locus associated with Bf*S07 in a tumisian population. Tissue Antigens, 35, 1990, pp
-219.
HARVEY , Colten. Molecular regulacion of complement gene expression. Progress in lmmunology, 1986. pp 314— 324.
HELLMAN, U. et al. primary sequence differences berwenn Chido an
Rodgers variants of triptic C4d of the human complement system FEB5.
Vol 170, n2, 1984, pp 254-258.
HOLME, E. et la. Quantiation of human C4A and C4B , in serum and plasma by enzyme linked inmunoabsorbent assay. lmmunogenetics 27,1988, pp 295- 297.
INSEMAN, D. and YOUNG , J. The molecular basis for the diference ininmune hemolysis activity of the Chido and Rodgers isotypes of human complement component C4. The Journal of lmmunology, vol 132, n 6,1984, pp 3019— 3027.
KEYEUX, G. Poblaciones negras de Colombia: una primera aproximación a su estructura molecular. América Negra , n5, Bogotá, 1993. pp 21 -33.
KEYEUX, G. Molecular análisis of the IGHA and MHC class lll región genes in one family with lg-A and C4 deficiencies . Experimental and
Clinical lmmunogenetics, 7, 1990 pp 170—180.
KOBAYASHI, K. et al. Associations betwenn restriction fragment length
polymophisms delected with a probe form human C4 and allotyp of C4B5 allele Human lmmunology, 29. 1990, pp 3-13.
KYNOSHIA, Taroh . Biology of complement the overture lmmunology
Today ,vol 12 n, 9. 1991,pp 291-2941.
KRAMER, J, et al Complement , C2. C3, C4, and factor B allele distribution in the gipsy population in Hungary lrmnunology Letters 24, 1990, pp 11—12.
LEWONTIN, R. La diversidad humana . Editorial Labor , Barcelona, 1984, pp 29—52.
LUNDWALL A. Et al. Isolation of tryptic fragments of human C4 expressing Chido and Rodgers antigens. Molecular lmmunology, vol 19,n12. 1982,pp 1655- 1665.
MARGNI R. lmmunologia e lnmunoquimica . Editorial Panamerican,
cuarta edición , Buenos Aires. 1989, pp 146—189.
MAUFF. G, et al. C4 nomenclature stament C4. Complement and lnflammation 7. 1990, pp261-268.
MAUFF , G, and BRAUN - STIL WELL M. Relative electrophorerie migration distances for the classification of C4 allotypes. Complement and inflammation 7. 1990, pp 277-281.
MAUFF , G. et al. The C4 beta chain. Evidence for a genetically determined polymorphis, Hum Genet 64, 1983, pp186-188.
MAUFF, G. et al . Revise numenclature for human complement component C4 Bulletin of the World Heahh Organization . 70 , 4, 1992, 531-532.
MAUFF, G. Application of the MHC class III complement markers to
population genetics 1987.
MAYER, Manfred Complement de overture lmmunology Today , vol 12, a
1991,pp291-294.
MEYER 0, et al. Genetic deficiency of C4, C2 or Clq and lupus síndromes.
Association with anti- Ro (ss- a) antibodies . Clin Exp. lnmunology, 62,
678—684.
MAYER M. inmunología , segunda edición. Prensa Cientifica, Barcelona,
165—177.MCLEAN, K el al. Characterization of two hibrid C4 allotypes and B*3 by electrophoretic, secological and restriction fragment lengh polymorphysm analyse tissue antigens 35. 1990. pp 75—81.
MEVAD, B. OLAISEN. D. and TEISBERG, P. Electroporetic of human C4
is due to charge diferences in the alfa chain, presumably in the C4d fragment scand. .J. inmunol. 14, 1981, pp 303-307.
MORGAN, P. and WALPPORT, M. Complement deñciency and disease,
vol 12, n 9, 1991.pp 301—306.
MOULDS, J. and DEJONGH, R. influence of C4B null genes on cytomegalovirus antibody titers in healthy blood donor. Transfusion, 32 1992, pp 145—147.
MÚLLER, J. and BIRD, C. isolation and description of the fourth component of human complement. J. Exp. Med. 1963, pp 447—467.
OLAISEN, B. et al. The C4 system human Genet N 50, 1979. pp 187—192.
OLAISEN, B. et al. Human complement C4 locus is duplicated on some
chromosomes nature, vol 279, 1979, pp 736-737.
O'NEILL YOUNG YANG,S. and TEGOLI, J. Chido and Rodgers blood
groups are distinct antigenic components of human complement C4 Nature ,vol 273, l978,pp 668—670.
O'NEILL,G. and DUPONT, B. Serun C4 levels Chido—Rodgers and allotypes of C4 component complement transplantion procedings.
O'NEILL, G. C4 Polymorphism usse of a monoclonal antibody to distinguis C4A and C4B locus products vox sang 47, 1984, pp 362—365.
PALSDOTTIR A. Et al Gene organization of haplotypes expressing two
diferent C4A allotypes Hum Genet 76, 1987, pp, 220-224
PALSDOTTIR A. Et al . Correlation between a DNA restrictioon fragment
length polymorphism and C4A6 protein Nature , vol 306 , 1983. pp, 615- 616.
PARTAMEN J. And KOSKIMIES 8. Human MHC Clase III genes Bf and
C4 polimorphism complotypes and association with MHC class I genes in the finnnish population Hum Hered 36. 1986.pp 269-275.
PARTAMEN J, and CAMPBELL, D. Restriction fragment analysis of non-
deleted complement C4 and genes sugesis point mutations in C4A null
alleles but gene conversions in 018 null alleles inmunogenetics 30 1989
pp 520-523.
PORTER. R. Complement polymorphism the mayor bistocompatibility
conplement and associated diseases A speculation Mot Biol Med
l,1983.pp161—168.
PRENTICE H, et al C4B gene polymorphism detected in a human cosmíd
clone inmunogenetics 23. 1986.pp 274—276.
REDMANN.V. et al Quantitation of the human component C4 definition of C4QO alleles and C4A duplications vox sang 63 1992.pp 117—123.
REILY.B,LEVINE, P .and SKANES V. Journal of Inmunology. Vol 147,
n 9. 1991,pp 3018-3023.
REID R et al Complement system proteins which interact with C3b
Inmunology today. Vol 7,n 7 , 8,pp230-234.
REGUEIRO, J. And LOPEZ C lnmunologia,biologia y patología del
sistema inmune, Editorial Interamericana , Madrid 1996,pp 25-30.
REGUEIROJ.ARNAIZ-VILLENA, A Human MCH class III (BF ,C2,C4)
genes and GLO; their association with other HLA anti gens and extended
haplotypes in the spanish population. Tissue antigens, 1987, 31 , 14-25.
RITTNER C. and BERTRAMS J. On the signiñcance of C2, C4, and
factor B polimorphism in disease Hum Genet vol 56, 1981, pp 235-247.
RITTNER c. and MAÚFF G. C4 polymorphism Histocompatibility, 1984,
pp 318-324.
ROITT, Ivan Inmunología, fundamentos , séptima edición, Editorial
Panamericana , Buenos Aires, 1994, pp 22 57—62.
ROJAS W. Inmunología, Editorial colima ,tercera edición , Medellin,
pp 64—78.
ROJAS W. Inmunología (CIB) Centro de investigaciones biológicas,
octava edición,Medellín 1990,pp 184-200
ROSS M et al A molecular basis for the two locus model of human complement C4.Nature, vol 298, 1982, pp 854—856.
ROOS. M. Et al Rodger (Rg) and chido (Ch) determinants on human
C4 Characterization of two C4,BS Subtypes one of which cotains Rg
and Ch determinants. The journal of Inmunology voll33, n 5, 1984, pp.
—2640.SCHNEIDER P, et al polymorphism of the human complement C4 and steroid 21—hidroxylase genes J. Clin invest vol 78, 1986, pp650-657.
SCHIFFERLI, .J. et al. Complement. Mediated adherence of immune
SCHEREIBER R and MÚLLER J fourth component of the interchain
disu1ñde bonds of the fourth component of human complement (C4)
evidence based on the liberation of fmgments secondary to ,thiol-disul-
tide interchange reactions journal of lmmunology vol 136, a 11. 1986,
pp 41 52—4157.
SILVE, J , et al structural polymorphism of human Dr antigens Nature
, 1979.pp 436—437.
SIM E and¿CROSS Phenotyping of human complement componet C4
class Ill HLA antigen Biochem J 239,1986,PP 763-767.
STEVER M,et al An estimate on the frequency of duplicatcd haplotypes
and silent alleles of human C4 protein polymorphismó. lnvestigations
in healthy Caucasoid families. Tissue antigens. 33, 1989, pp 501 -510.
TAROH, K. Biology of complement the overture inmunology today. Vol
, n9 1991,pp 291-294.
TEISBERG, P. et al. The genetic polymorphism of the fouth component
of human complement: Methodogical aspects and a presentation of link-age an association data relevant to its localization in the HLA region.The Journal of Experimental Medicine. Vol 146, 1977, pp 1380-1389.
TERTIA, K. et al. The structural basic of the multiple forms of human
complement component C4. Cell, vol 36, 1984, pp 907-914.
TILLEY, C. ROMANS, D. CROOKSTON, M. Localization of Chide and
Rogers detenninants to the C4d fragment of human C4. Nature, vol 276,
,pp 713—71.
TIMOTHY, C. et al. Evolution of the complement system. lnmunology
Today. Vol 12, n 9, 1991 , pp 295-299.
TRUEDSSON, Lennart, et al. Quantitativa variation of C4 variant pro-
teins associated whit many MHC haplotypes. lnmunogenetics, 30, 1989,
pp 414—412.
YUNG, YU. C. The complete exon-intron structure of a human comple-
ment component C4A gene. Journal of inmunology. Vol 146, n 3, 1991 ,
Pp 1057 — 1066.